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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Am J Transplant. 2020 Jul 5:10.1111/ajt.16148. doi: 10.1111/ajt.16148

FIGURE 2.

FIGURE 2

Normothermic reperfusion triggers intravascular accumulation of tubular-cell-derived fibrinogen. A, Representative images of biopsies from human kidneys sampled during cold storage (upper panels) demonstrating positive stain in tubular epithelia in both martius scarlet blue (MSB; left; pink stain) and immunofluorescence (right; fibrinogen/fibrin[ogen]—red; Ulex—white; Dapi—blue). A repeat biopsy after 30 min of normothermic machine perfusion (NMP) in the same organ depicts the migration of the positive stain to the vasculature (lower panels). B, Fibrin(ogen) levels in perfusate as measured by ELISA in technical triplicate for samples taken before and during NMP. Stars indicate samples below limit of detection. C, Urine fibrin(ogen) levels in human kidneys measured in technical triplicate at 30 min of NMP. D, Representative images from a clinical cohort of kidneys during cold storage demonstrating positive MSB stain in tubular epithelia with MSB stain (top). A repeat biopsy in the same organ taken 30 min posttransplant depicts the migration of the positive stain to the vasculature (bottom). Scale bars represent 100 μm