Table 3.

Summary of VO_2,average (μmol/g tissue/min) and cerebral blood flow (ml/g tissue/min) results based on washout in brain.

Individual animal	VO2,average Left & Right Muscle ROI	VO2,average Left & Right Brain ROI	CBF Left Brain ROI	CBF Right Brain ROI
rat A	1.17	1.19	0.27	0.27
	1.19	1.17	0.28	0.26
rat B	1.21	1.04	0.28	0.25
rat C	1.19	1.16	0.26	0.28
	1.06	1.09	0.31	0.28
rat D	1.39	1.37	0.30	0.30
	1.15	1.14	0.28	0.26
Mean ± SD	1.19 ± 0.10**	1.17 ± 0.10**	0.28 ± 0.02**	0.27 ± 0.02**
rat E	1.14	1.11	0.41	0.33
	1.03	1.05	0.34	0.25
	1.02	0.95	0.29	0.29
rat F	0.64	0.91	0.32	0.33
rat G*	1.17	1.13	0.32	0.32
rat H*	0.74	0.73	0.46	0.38
Mean ± SD	0.96 ± 0.22**	0.98 ± 0.15**	0.36 ± 0.06**	0.32 ± 0.05**
Overall average of both N2 & N2O	VO2,average (n=13) 1.08 ± 0.20 μmol/g body/min	VO2,average (n=13) 1.08 ± 0.16 μmol/g body/min	CBF (n=26) 0.30 ± 0.05 ml/g tissue/min

^*This subgroup of 2 animals was acquired at a higher temporal resolution (10s per 3D CSI volume) and with lower spatial resolution protocol.

^**p<0.05 significant population difference between N₂ (rats A-D) and N₂O (rats E-H) groups (unpaired t-test).