Figure 4: Nitrotyrosine in zebrafish hair cells.

(A) Immunolocalization with anti-nitrotyrosine indicated that cisplatin treatment increased the nitrotyrosine levels (purple) in the hair cells of 5dpf stage larvae. Parvalbumin (green) was used to stain the hair cells. Images are representative of six replicates. Scale bar= 5 μm. (B) Quantification of the staining intensity in neuromasts located in the anterior, middle, and posterior regions indicated a significant increase in nitrotyrosine levels in cisplatin treated zebrafish. The results are expressed as mean ± standard error, n = 6, (***p<0.001, ****p<0.0001). (C) Analysis of the correlation between cisplatin-induced changes in hair cell viability and nitrotyrosine levels indicated a negative correlation (R²=0.74), n=6.