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Mean venous (blue) and stepwise venous-to-arterial (Arterial; red) perfusion culture conditions for 21 days. For the transition culture scenario, increases in pressure (solid lines) and volumetric flow (dashed line) were made at 3-day intervals with concatenate increases in pulsatility (not shown). Flow increased by 17.3% each step and pressure by 31.5%. Axial extension remained constant throughout.
