TABLE 3.

Possible assignments of major m/z peaks of Anti-JFL21 and the commercial fengycin standard^a.

Samples	Detected precursor ions (m/z)			Two fingerprint ions (m/z)	Possible assignmentb	Amino acid at position 6 and 10
	[M + H]+	[M + Na]+	[M + K]+
Anti-JFL21	1029.28	−	−	/	C13 iturin A	/
	1043.59	1065.57	−	/	C14 iturin A	/
	1057.60	1079.58	−	/	C15 iturin A	/
	1071.62	1093.60	1109.59	/	C16 iturin A	/
	1421.79	−	−	952.25, 1066.42	C14 fengycin A2	Ala6, Val10
	1435.81	−	−	952.35, 1066.44 966.37, 1080.48	C15 fengycin A2 C14 fengycin A1	Ala6, Val10 Ala6, Ile10
	1449.84	1471.82	−	952.30, 1066.41 966.31, 1080.40 980.34, 1094.45	C16 fengycin A2 C15 fengycin A1 C14 fengycin B2	Ala6, Val10 Ala6, Ile10 Val6, Val10
	1463.86	1485.84	−	952.32, 1066.44 966.35, 1080.46	C17 fengycin A2 C16 fengycin A1	Ala6, Val10 Ala6, Ile10
	1477.89	1499.86	−	952.43, 1066.50 966.37, 1080.48 980.39, 1094.51 994.39, 1108.52	C18 fengycin A2 C17 fengycin A1 C16 fengycin B2 C15 fengycin B1	Ala6, Val10 Ala6, Ile10 Val6, Val10 Val6, Ile10
	1491.91	1513.88	−	952.26, 1066.18 966.19, 1080.16 980.20, 1094.20 994.35, 1108.50	C19 fengycin A2 C18 fengycin A1 C17 fengycin B2 C16 fengycin B1	Ala6, Val10 Ala6, Ile10 Val6, Val10 Val6, Ile10
	1505.91	−	−	980.39, 1094.47 994.42, 1108.53	C18 fengycin B2 C17 fengycin B1	Val6, Val10 Val6, Ile10
fengycin standard	1447.81	1469.79	−	966.45, 1080.55 994.47, 1108.55	*C15 fengycin A1 *C13 fengycin B1	Ala6, Ile10 Val6, Ile10
	1463.81	1485.80	−	966.37, 1080.46 994.38, 1108.48	C16 fengycin A1 C14 fengycin B1	Ala6, Ile10 Val6, Ile10
	1477.84	1499.81	−	966.39, 1080.47 994.40, 1108.49	C17 fengycin A1 C15 fengycin B1	Ala6, Ile10 Val6, Ile10
	1491.85	1513.83	−	966.34, 1080.45 994.33, 1108.44	C18 fengycin A1 C16 fengycin B1	Ala6, Ile10 Val6, Ile10
	1505.87	1527.85	1543.83	994.34, 1108.46	C17 fengycin B1	Val6, Ile10

^a−, no detected; /, not tested or studied; *, indicates one double bond in the fatty acid chain. ^bPossible assignments based on Pathak et al. (2012), Torres et al. (2015); Yang et al. (2015), and Sarwar et al. (2018).