FIGURE 10.

qRT-PCR based expression analysis of chimeric FTST variant relative to the expression of the internal housekeeping genes EF1α and L21. Expression level of the FTGT variant was determined with a primer pair targeting the catalytic domain of the rat ST. FTST-expression levels were normalized line internally against the expression levels of the two housekeeping genes EF1α and L21. The expression analysis was performed on eight candidate lines, the corresponding parental line GMC_GT25 and the parental line P, expressing none of the sialic acid pathway genes. The relative expression levels are expressed as 2^(–ΔCT), where ΔCT = CT transgene – CT housekeeping genes. Bars represent mean values ± SD from n = 3.