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. 2020 Dec 18;11:602444. doi: 10.3389/fmicb.2020.602444

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Copyright © 2020 Low, Böhnlein, Sprotte, Wagner, Fiedler, Kabisch and Franz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of unspecific staining in A. johnsonii. (A) Standard adsorption assay of PMBT14 using A. johnsonii L2-215 and host P. fluorescens DSM 50090. Bar graphs depict the amount of unadsorbed bacteriophage (n = 12 from four experiments). One-way ANOVA was used to compare the means between the groups (2 degrees of freedom; F-value 13.968; *p < 0.05; **p < 0.01; ***p < 0.001). (B) Staining intensity of Tween 20 treated (+Tween 20) PMBT14-Syto 13 versus original untreated PMBT14-Syto 13 (–Tween 20). (C) Histograms (left) depicting the effect of increasing concentrations of sodium azide on the fluorescence of P. fluorescens DSM 50090 and A. johnsonii L2-215 after incubation with PMBT14-Syto 13. Bar graphs (right) depict the corresponding mean fluorescence intensity with SEM (n = 3).