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. 2020 Dec 18;11:595726. doi: 10.3389/fpls.2020.595726

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Copyright © 2020 Lee, Carroll, Crossett, Connolly, Batarseh and Djordjevic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The FA-CTLC method preferentially identifies peptides with TMD motifs. (A) A comparison of the total TMDs identified numbers from both purification methods. The FA-CTLC methods identified 9.3-fold more TMDs than the UR-TLC method. (B,C) The peptides identified in the MTR_7g005910 transporter of Medicago after using the FA-CTLC (B) or UR-TLC method (C). The peptides that were identified by MS were colored in black. The schematic diagrams were made using the Protter online tool (Omasits et al., 2014) (http://wlab.ethz.ch/protter/start/).