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. 2020 Dec 18;11:595726. doi: 10.3389/fpls.2020.595726

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Copyright © 2020 Lee, Carroll, Crossett, Connolly, Batarseh and Djordjevic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The predicted subcellular location of the proteins identified after using FA-CTLC or UR-TLC. (A,B) Between 73 and 75% of the proteins identified in the MM preparations were cytoplasmic proteins. Of the proteins identified to be TMPs, there was no significant difference in the identity of the proteins predicted to reside in the nuclear, chloroplast, or mitochondrial membranes. A t-test confirmed a significance difference (p < 0.05, n = 3) between the two methods in identifying proteins where the subcellular location could not be assigned (the “unassigned” category).