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. 2020 Dec 31;10:22436. doi: 10.1038/s41598-020-80288-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Colorectal cancer cells expressing NAT2_5 and NAT2_14 but not NAT2_7 are sensitive to APA treatment. (A–C) Quantification of NAT2 catalytic activity in RKO clones expressing (A) NAT2_5, (B) NAT2_7 and (C) NAT2_14 enzymatic variants. The velocity at which APA becomes acetylated was measured by LC–MS/MS at different concentrations. A representative experiment is shown for each enzymatic variant. (D–F) Dose response for APA in RKO clones expressing the (D) NAT2_5, (E) NAT2_7 and (F) NAT2_14 enzymatic variants. Cell viability was measured by a MTT assay after 72 h. The mean and S.D. of three independent experiments is shown. Data were analyzed using a two-way ANOVA. **p < 0.01 and ****p < 0.0001.