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. 2021 Jan 1;8:7. doi: 10.1038/s41438-020-00437-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The growth phenotype of O. pumila hairy roots at different growth stages (scale bars: 1 cm). b The biomass of O. pumila hairy roots at different growth stages. Error bars represent the SD of three biological replicates. c The production of camptothecin in hairy roots and liquid medium was detected by HPLC. Error bars represent the SD of three biological replicates. d The transcription of five camptothecin biosynthetic genes at different growth stages was detected by qRT-PCR analyses. The transcriptional expression level of each gene at 10 days was set to 1. The OpActin gene was used as the internal reference gene. Error bars represent the SD of three technical replicates.