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. 2021 Jan 1;8:7. doi: 10.1038/s41438-020-00437-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a O. pumila explants on B5 medium. b O. pumila stems precultured on B5 medium. c Hairy roots differentiated from infected S. miltiorrhiza explants. d Isolated monoclonal hairy roots. e Hairy-root monoclones cultured in B5 liquid medium. f, g The relative transcript levels of OpWRKY2 in the OpWRKY2-OE (f) and OpWRKY2-SRDX (g) transgenic hairy-root lines were detected by qRT-PCR. The pCAMBIA2300⁺ empty vector was used as a control. The average expression level of OpWRKY2 in the two control hairy-root lines was set to 1. The OpActin gene was used as the internal reference gene. Error bars represent the SD of three technical replicates.