Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 1;8:7. doi: 10.1038/s41438-020-00437-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — a Diagram of the OpTDC promoter fragment and the sequence of a subfragment containing a W-box motif or mutant W-box motif. b Electrophoretic mobility shift assay (EMSA) indicating that OpWRKY2 binds to the W-box in the OpTDC promoter. pCold-HIS protein without OpWRKY2 was used as the control. c Dual-luciferase (Dual-LUC) assays showed the activation effect of OpWRKY2 on the OpTDC promoter. The OpTDC promoter and OpTDC promoter containing a mutant W-box motif were fused to the firefly luciferase reporter gene, and the promoter activity was determined by a transient Dual-LUC assay in N. benthamiana. The relative LUC activity was normalized to that of the reference Renilla (REN) luciferase. Error bars indicate the SD (n = 3). Student’s t-test: **P < 0.01; NS no significance. d Yeast one-hybrid (Y1H) assay indicating that OpWRKY2 binds to the W-box in the OpTDC promoter. Yeast cells transformed with different combinations of constructs were grown on SD/-Ura/-Trp/+X-gal medium. Pictures were taken after 4 days of incubation at 30 °C. Y1H assays were repeated three times.