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. 2020 Dec 31;10:22431. doi: 10.1038/s41598-020-80087-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Delta CT values of Morc1 mRNA analyzed in mPFC brain region. Separated dams were expressing significantly lower levels of Morc1 mRNA as shown in significant higher CT values. Means ± SEM are presented for n = 15 controls and n = 13 separated dams. *P < .01. (B) Delta CT values of Nr3c1 mRNA analyzed in hippocampus brain region. Means ± SEM are presented for n = 8 controls and n = 8 separated dams. (C, D) Serum analyses of neurotransmitters glutamate for n = 14 controls and n = 15 separated dams and GABA for n = 15 controls and n = 14 separated dams after MS. Separated dams showed higher concentrations of both neurotransmitters but only GABA demonstrating significant differences. Means ± SEM are presented. Data was analyzed using a One-Way ANOVA.