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. 2021 Jan 1;8:6. doi: 10.1038/s41438-020-00436-4

Fig. 3. Acquisition of transgenic plants.

Fig. 3

a Diagram of the pBI121-CmWRKY15-1 and pRNAi-CmWRKY15-1 vectors. CaMV 35 S, promoter; NOS, nopaline synthase terminator; nptII, neomycin phosphotransferase gene; CmWRKY15-1, target fragment; LB and RB, left and right borders of the T-DNA; SacI, XbaI, BamHI, and HindIII, cloning sites; A1, antisense fragment; S1, sense fragment; PDK, intron. b PCR-based analysis of kanamycin-resistant transgenic plants. M, DNA ladder (DL 2000); OE-1 and OE-2, overexpression transgenic plants; RNA1 and RNA2, RNA interference transgenic plants. c Relative expression levels of CmWRKY15-1.