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. 2020 Dec 31;10:22458. doi: 10.1038/s41598-020-80444-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Antimicrobial resistance (AMR) abundance of the samples. (a) Violin plot representing the distribution of the total AMR fragments per kilobase per million fragments (FPKM) per sample, grouped by type and source. The horizontally jittered dots represent the FPKM of the samples. (b) The AMR abundance diversity ( $β$ -diversity) of the samples. It is plotted on the first two axes of principal coordinate analysis (PCoA) performed on Bray-Curtis distance which was calculated using the relative abundances of contigs harbouring ARGs. The symbols show the type, the colours the source, while the numbers correspond to the sequence number in the Sample ID. Some samples (k_s_01, k_s_04, k_s_05, k_s_06, k_p_08) are not shown as their ORFs did not meet filtering criteria.