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. 2020 Dec 31;10:22452. doi: 10.1038/s41598-020-80253-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Magnetic manipulation of HT-SOS1cat-Clover-transfected SH-SY5Y cells upon the injection of HTL-MNPs. The fluorescence of HT-SOS1cat-Clover is shown in the left column (Supplementary Video S5), and the fluorescence of the HTL-MNPs is shown in the right column (Supplementary Video S6). For better visualization of the corresponding fluorescence intensities, a rainbow color scheme was applied, with red corresponding to high, yellow to medium, and blue to low fluorescence intensities (for HT-SOS1cat-Clover: Supplementary Video S7; for HTL-MNPs: Supplementary Video S8). The insets show magnifications of the ROI. (a,b) At 0 min 00 s, HT-SOS1cat-Clover was distributed homogeneously in the cytoplasm. (f,g) HTL-MNPs were slightly accumulated on the left and right sides of the nucleus of the cell in the ROI. (h) At 3 min 50 s, HTL-MNPs were attracted towards the magnetic tip and accumulated at the proximal plasma membrane. (c) Similar behavior was seen for HT-SOS1cat-Clover, although the change in fluorescence intensity was weaker. (i,d) When the tip was moved at 4 min 40 s, HTL-MNPs moved into the small neurite and an increase of HT-SOS1cat-Clover fluorescence was observed. (j,e) After the tip was removed, HTL-MNPs and HT-SOS1cat-Clover began to diffuse in the next two minutes. Scale bars correspond to 20 µm.