Fig. 6. Role of SmMYB1 in promoting anthocyanin accumulation in S. miltiorrhiza transgenic hairy root lines.

A, B Anthocyanin contents and the appearance of extracts from transgenic hairy root lines. Fold changes of the relative anthocyanin contents in transgenic hairy roots were normalized to the contents in the control. Control: empty vector; SmMYB-OE: SmMYB overexpression; SmMYB-SRDX: SmMYB-SRDX repression. A Expression profiles of genes involved in anthocyanin biosynthesis. Fold changes of the relative gene expression level in transgenic hairy roots were normalized to the level in the control. B Activation of CHI and ANS expression, as revealed by dual-LUC assays. The effector construct contained SmMYB1 driven by the CaMV 35S promoter. The promoters of candidate genes (CHI, ANS) fused to LUC were used as reporter constructs. An effector vector not carrying SmMYB1 served as a negative control. The error bars indicate the standard errors of the means. The asterisks indicate significant differences according to t-tests at two different significance levels (**P < 0.01; *P < 0.05). The experiment was replicated three times