Fig. 7. SmMYB1 interacts with SmMYC2 to promote CYP98A14 expression.

A BiFC assay; scale bars, 50 µm. B Y2H assay. AD: pGADT7 vector; BD: pGBKT7 vector. C Coexpressing SmMYB1 and SmMYC2 enhanced the activation of the reporter construct, as revealed by dual-LUC assays. The effector constructs contained SmMYB1 or SmMYC2 driven by the CaMV 35S promoter. proCYP98A14::LUC was used as the reporter construct. Effector vectors not carrying SmMYB1 or SmMYC2 were used as negative controls. Fold changes of the relative LUC activity were normalized to the activity of the control. The asterisks indicate significant differences according to t-tests at two different significance levels (**P < 0.01; *P < 0.05). The experiment was replicated three times