Fig. 1. Soft rot symptoms on Zantedeschia (calla lily) leaf discs taken from different cultivars and infected with Pectobacterium brasiliense (Pb): Z. aethiopica, ZA; “Captain Romance”, CR; “Florex Gold”, FG; “Hot Shot”, HS.

A Symptoms were determined as the percentage of macerated tissue out of total leaf disc area, 24 h post inoculation with 10 µl of bacterial suspension (10⁸ CFU/ml, OD₆₀₀ = 0.1), and incubation at 28 °C. Data represent means ± SE of three independent experiments with four plates taken from different plants containing ten replicates for each cultivar. Treatments labeled differently are significantly different (P < 0.05). B Representative pictures of one plate of each of the tested cultivars. C Confocal images of ZA and CR inoculated as above with Pb expressing GFP or with distilled water (control). The point of inoculation was viewed under a fluorescent microscope with a GFP filter (left) and a UV filter (right), 24 h post inoculation