Fig. 2. Microscopic examination of leaf and petiole morphology of Zantedeschia aethiopica, (ZA) and the colored hybrid “Captain Romance” (CR).
A Transverse leaf section of ZA and the hybrid CR stained with safranin-fast-green viewed under a light microscope. Resolution ×10, black bar 100 μm. B Transverse petiole section of ZA and the hybrid CR stained with toluidine blue and viewed under a light microscope. Resolution ×5, white bar 500 μm. C Air-space ratio was calculated using ImageJ software, as a percentage of section area. Data represent means ± SD of six fully opened second leaves. Treatments labeled with * are significantly different (P < 0.01). D Scanning electron microscopy (SEM) images of adaxial (upper) and abaxial (lower) leaf surfaces of ZA and CR. Resolution ×400, white bar 50 μm