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. 2020 Dec 18;11:613571. doi: 10.3389/fmicb.2020.613571

Figure 6.

Figure 6

Qpr6 affects the transport of catalases to the periplasm of D. radiodurans. (A,B) protein abundance of KatE1 (A), and KatE2 (B) in the whole cell lysates and periplasm of R1, Qpr6 KD and DR_1836 KD. Molecular weight is indicated in kDa on the left. SYPRO Ruby staining served as a loading control to indicate that the protein amount loaded in each lane is equivalent. The signal intensity from the bands was quantified by densitometry using TotalLab CLIQS. Error bars indicate standard errors of the mean (n = 2). (C,D) Catalase activities of the whole cell lysates (C) and the periplasmic fraction (D) in R1, Qpr6 KD, and DR_1836 KD. Error bars indicate standard errors of the means (n = 3). *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001 were considered as significant using the Student t-test while ns indicates non-significant compared to R1 (p > 0.05).