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. 2020 Dec 18;11:614923. doi: 10.3389/fneur.2020.614923

Figure 1.

Figure 1

Loss of GABAergic interneurons during the early phase of kainate-induced epileptogenesis. (A) Representative confocal images of parvalbumin (PARV) immunoreactivity in the hippocampal CA1 region and dentate gyrus (DG) of mice injected with kainate 5 days and 14 days before. (B) Quantification of the number of parvalbumin-positive neurons in an area of 582 × 582 × 40 μm within the hippocampal CA1 and DG region below the injection site. N = 6 slices from 3 animals for each time point and area. (C) Quantification of the number of GABA-positive neurons (GABA staining shown in Figure 3) in the ipsi- and contralateral hippocampus of sham and kainate injected animals. Cells were counted in an area of 246 × 246 × 40 μm within the hippocampal CA1 and DG region below the injection site. N = 5 slices from five animals (GABA) for each time point, area and condition. Str. pyr. = Stratum pyramidale, Str. rad. = Stratum radiatum, dpi = days post-injection, n.d. = not detected. *ipsi- vs. contralateral significantly different, #significantly different from sham (p < 0.05, stratified two-way ANOVA followed by Tukey's test). Scale bar = 50 μm.