TABLE 1.
Strains and plasmids used in this study.
| Strain/plasmid | Characteristics | References |
| Pseudomonas aeruginosa | ||
| PAO1 | Wild-type; ATCC 15692 | Wei et al. (2011) |
| PAO1/pProbe-AT′ | PAO1 containing promoterless gfp reporter plasmid pProbe-AT′, Cbr | This study |
| PAO1/plasR-gfp | PAO1 containing reporter plasmid pProbe-lasR, Cbr | This study |
| PAO1/pmvfR-gfp | PAO1 containing reporter plasmid pProbe-mvfR, Cbr | This study |
| PAO1/prhlR-gfp | PAO1 containing reporter plasmid pProbe-rhlR, Cbr | This study |
| PAO1/pgacS-gfp | PAO1 containing reporter plasmid pProbe-gacS, Cbr | This study |
| PAO1/pgacA-gfp | PAO1 containing reporter plasmid pProbe-gacA, Cbr | This study |
| PAO1/pcyaB-gfp | PAO1 containing reporter plasmid pProbe-cyaB, Cbr | This study |
| PAO1/pcupE1-gfp | PAO1 containing reporter plasmid pcupE1-PUCPgfps, Cbr | This study |
| PAO1/ppprB-gfp | PAO1 containing reporter plasmid ppprB-PUCPgfps, Cbr | This study |
| PAO1/prpoS-gfp | PAO1 containing reporter plasmid pProbe-rpoS, Cbr | This study |
| PAO1/pdgcH-gfp | PAO1 containing reporter plasmid pProbe-dgcH, Cbr | Wei et al. (2019b) |
| Escherichia coli | ||
| DH5α | supE44ΔlacU169 (Φ80 lacZΔM15) recA hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Lab collection |
| S17-1 | thi pro hsdR recA; chromosomal RP4, Tra+ | Lab collection |
| Plasmids | ||
| pProbe-AT′ | Promoterless gfp reporter plasmid, Apr | Miller et al. (2000) |
| pProbe-lasR | Promoter sequence of lasR inserted into gfp reporter plasmid pProbe-AT′, Apr | This study |
| pProbe-mvfR | Promoter sequence of mvfR inserted into gfp reporter plasmid pProbe-AT′, Apr | This study |
| pProbe-rhlR | Promoter sequence of rhlR inserted into gfp reporter plasmid pProbe-AT′, Apr | This study |
| pProbe-gacS | Promoter sequence of gacS inserted into gfp reporter plasmid pProbe-AT′, Apr | This study |
| pProbe-gacA | Promoter sequence of gacA inserted into gfp reporter plasmid pProbe-AT′, Apr | This study |
| pProbe-cyaB | Promoter sequence of cyaB inserted into gfp reporter plasmid pProbe-AT′, Apr | This study |
| pProbe-rpoS | Promoter sequence of rpoS inserted into gfp reporter plasmid pProbe-AT′, Apr | This study |
| pcupE1-PUCPgfps | Transcriptional reporter plasmid of cupE1, Gmr | Wang et al. (2019) |
| ppprB-PUCPgfps | Transcriptional reporter plasmid of pprB, Gmr | Wang et al. (2019) |
| Primers | ||
| gacS-proFw | GGAATTCGCAGCATGATGTCCATCAGG | |
| gacS-proRv | CCCAAGCTTCTTGATGCCGAGATCCTTG | |
| gacA-proFw | GGAATTCGGAAGCAATCCTGGATCGTCG | |
| gacA-proRv | CCCAAGCTTGGTGCGTACCAGATCGTGGTC | |
| cyaB-proFw | GGAATTCCTGGGTGATGCTCAAGGACG | |
| cyaB-proRv | CCCAAGCTTGGTAGGCTTCATGCGCTGGA | |
| rpoS-proFw | GGAATTCGCGAGCGGTACTCTGATCG | |
| rpoS-proRv | CCCAAGCTTCTCCAGGAGGAGCACTTC | |
| mvfR-proFw | GGAATTCCTACACCTGAAGGCGCAAC | |
| mvfR-proRv | GGGGTACCGATGACCTGGAGGAACATG | |
| rhlR-proFw | GGGGTACCCACTGGGAGCCTTGCTGC | |
| rhlR-proRv | CCCAAGCTTCATCTCGCTACGCAAACC | |
| lasR-proFw | GGAATTCGTGTGACTGGGTATTCAGTTCG | |
| lasR-proRv | CCCAAGCTTCGTCAACCAAGGCCATAGC | |