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. 2021 Jan 1;7(1):eabe5357. doi: 10.1126/sciadv.abe5357

Fig. 7. S100A8/A9 interacts with transcription factors and activates transcription upon artificial recruitment.

Fig. 7

(A) Western blot of S100A9-Flag immunoprecipitates of the indicated proteins in nuclear extracts from cells treated with EtOH (E) or TAM (T); lactate dehydrogenase (LDHA) is used as a cytoplasmic marker. (B) Transcriptional activity (normalized luciferase levels) in MCF7 cells electroporated with the indicated Gal4 DNA binding domain (DBD) protein derivative and the reporter construct containing five tandem repeats of the Gal4 binding site upstream of the core Drosophila HSP70Bb TATA-box promoter. Data are means ± SEM; n = 4; ***P < 0.001 compared with the Gal4DBD group; t test.