Table 3.
Precision, accuracy, matrix effect and extraction recovery for the four analytes in rat plasma.
| Compounds spiked concentration (ng/mL) | Intra-day (n = 5) |
Inter-day (n = 15, 5 replicates per day for 3 days) |
Matrix effect (n = 6) |
Extraction recovery (n = 6) |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Measured concentration (ng/mL) | Precision (RSD,%) | Accuracy (RE,%) | Measured concentration (ng/mL) | Precision (RSD,%) | Accuracy (RE,%) | Mean (%) | RSD (%) | Mean (%) | RSD (%) | |
| Shanzhiside | ||||||||||
| 10 | 10.50 ± 0.44 | 4.2 | 4.1 | |||||||
| 20 | 19.74 ± 0.46 | 2.3 | −2.3 | 20.82 ± 1.19 | 5.7 | 4.7 | 83.8 | 7.9 | 53.8 | 8.6 |
| 100 | 103.31 ± 6.83 | 6.6 | 0.6 | 101.05 ± 6.39 | 6.3 | 7.3 | 83.2 | 8.5 | 54.6 | 4.6 |
| 160 | 158.62 ± 5.83 | 3.7 | −0.6 | 161.34 ± 8.64 | 5.4 | −2.5 | 85.7 | 7.4 | 60.8 | 4.7 |
| Genipin-1-gentiobioside | ||||||||||
| 1 | 1.15 ± 0.03 | 2.5 | 13.7 | |||||||
| 2 | 2.04 ± 0.10 | 5.0 | 2.9 | 1.99 ± 0.10 | 3.2 | 1.1 | 81.2 | 8.6 | 98.8 | 8.0 |
| 500 | 540.63 ± 17.18 | 3.2 | 7.4 | 533.35 ± 24.73 | 4.6 | −0.3 | 87.7 | 6.5 | 99.7 | 5.3 |
| 800 | 810.89 ± 32.72 | 4.0 | 0.2 | 820.64 ± 26.02 | 5.0 | 4.1 | 83.3 | 3.4 | 84.9 | 9.0 |
| Geniposide | ||||||||||
| 1 | 1.11 ± 0.04 | 4.0 | 8.8 | |||||||
| 2 | 1.96 ± 0.24 | 12.2 | −10.1 | 2.02 ± 0.20 | 9.8 | 7.0 | 82.0 | 3.7 | 89.9 | 6.0 |
| 2000 | 2025.19 ± 164.14 | 8.1 | −0.1 | 2036.98 ± 140.32 | 6.9 | −0.1 | 89.2 | 5.1 | 87.7 | 8.5 |
| 3200 | 3172.78 ± 116.84 | 3.7 | −1.6 | 3271.67 ± 155.95 | 4.8 | 2.3 | 82.0 | 5.0 | 92.5 | 5.1 |
| Genipin | ||||||||||
| 1 | 1.15 ± 0.04 | 3.5 | 16.4 | |||||||
| 2 | 2.03 ± 0.06 | 2.8 | 3.1 | 2.06 ± 0.11 | 3.9 | 7.8 | 88.1 | 5.5 | 71.0 | 9.4 |
| 1000 | 1012.98 ± 23.61 | 1.8 | 2.8 | 1042.44 ± 75.04 | 7.2 | 3.4 | 88.4 | 8.6 | 70.0 | 3.2 |
| 1600 | 1602.72 ± 23.61 | 1.5 | 1.3 | 1603.35 ± 61.85 | 5.5 | 1.4 | 82.5 | 4.4 | 67.6 | 7.9 |