Fig. 4.

2-HB attenuates AP-induced cellular toxicity. (A) BRL3A cells, (B) AML12 cells, and (C) mouse primary hepatocytes were treated with AP alone or in combination with 5–40 mM 2-HB for indicated periods, and cell viability was measured with CCK-8 kit (n = 6). (D) Representative photographs of BRL3A cells treated with AP alone or in combination with 5–40 mM 2-HB for 48 h (The magnification of photographs is 100×). (E) Cellular GSSG/GSH was analyzed in primary hepatocytes treated with AP alone or in combination of 5 mM 2-HB for 24 h (n = 3). Data were mean ± S.E.M. *P < 0.05, **P < 0.01.