Fig. 5.

2-HB treatment protects AP-induced acute liver injury in mice. Mice were intraperitoneally injected with 2-HB (250 mg/kg, once a day) for 4 days and administered with a single dose of AP (400 mg/kg) 30 min after the last injection of 2-HB. (A–B) Serum ALT, AST, and liver histology in mice 24 h after AP treatment (n = 5–7). *P < 0.05, **P < 0.01. (C–D) Serum concentrations of AP and 2-HB quantified with LC-MS/MS in mice treated with AP and/or 2-HB (n = 6). *P < 0.05, **P < 0.01 compared to the same time point between the two groups. Data were mean ± S.E.M.