Table 3.
Detection of cys by HPLC.
| Derivatization agent | Flow rate (mL/min) | Stationary phase | Mobile phase | T (°C) | Elution time (min) | Limit of detection | Refs. |
|---|---|---|---|---|---|---|---|
| CMQTa | 1 | C18 (5μm; 4.6 mm × 150 mm) | Gradient elution or isocratic elution (trichloro acetic acid and acetonitrile) | 25 | 9 | 0.1 μM | [48] |
| mBBrb | 1.5 | C18 (5 μm; 4.6 mm × 150 mm) | Gradient elution (methanol, acetic acid and water) | RT | 12.5 | Not defined | [40] |
| 1.5 | C18 (3 μm; 4.6 mm × 150 mm) | Acetonitrile | RT | 4.3 | 50 nM | [41] | |
| 0.3 | C18 (5 μm; 2.1 mm × 100 mm) | Water: methanol (65:35) | – | 11 | 2 nM | [42] | |
| SBD-Fb | 1 | C18 (8–10 μm; 3.9 mm × 300 mm) | Gradient elution (methanol and sodium acetate) | RT | 10 | 0.07 pmol | [43] |
| 0.3 | C18 (5μm; 2.0 mm × 250 mm) | Phosphate buffer: CH3CN (96:4) | 30 | 5 | 0.47 μM | [44] | |
| NPMb | 1 | C18 (5μm; 4.6 mm × 250 mm) | Acetonitrile: water (70:30) | RT | 10 | 0.01 nM | [49] |
| DAABD-Clb | 0.6 | C18 (2 nm, 4.6 mm × 150 mm) | Gradient elution (water, acetonitrile and trifluoroacetic acid) | 50 | 6.4 | 154 fmol | [51] |
| ABD-Fb | 1 | C18 (3μm; 3.9 mm × 150 mm) | 2.5% methanol and ammonium acetate | – | – | – | [52] |
| ACQb | 0.3 | C18 (5μm; 2.1 mm × 150 mm) | Gradient elution (sodium acetate and trimethyl- amine, acetonitrile and water) | RT | 29 | 0.77 pmol | [53] |
CMQT: 2-chloro-1-methylquinolinium tetrafluoroborate, mBBr: monobromobimane, SBD-F: ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate, NPM: N-(1-pyrenyl) maleimide, DAABD-Cl: 7-Chloro-N-[2-(dimethylamino)ethyl]-2,1,3-benzoxadiazole-4-sulfonamide, ABD-F: 4-Fluoro-7-sulfamoyl benzofurazan, ACQ: 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate, RT: room temperature, T: temperature. a HPLC coupled with UV detection; b HPLC coupled with fluorescence detection.