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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: J Thorac Oncol. 2020 Oct 7:S1556-0864(20)30765-6. doi: 10.1016/j.jtho.2020.09.019

Figure 2. ERBB2 plays an important role in cells expressing EGFR L861Q.

Figure 2.

(A) Ba/F3 cells stably expressing the indicated cDNAs were cultured without IL-3 and counted daily. Data are representative of three independent experiments conducted with duplicate determinations (mean ± SE). (B) Lysates were extracted from 3T3 cells stably expressing the indicated cDNAs and subjected to western blot analysis. (C) Cells were serum-starved for 6 h, lysed, and then probed by western blot. (D) Isogenic stable cell lines were serum-starved for 6 h, lysed, and subjected to co-immunoprecipitation followed by western blot analysis. (E) HBECp53 cells were transfected with the construct indicated and incubated for 48 h after which lysates were extracted and subjected to western blot analysis.