Generation of transcripts with exon skipping events. Sequence A is a reference transcript with three exons, a homozygous SNV, and a heterozygous SNV. The length of the cassette exon is l. A homozygous transcript B is generated from A by adding the homozygous SNV. An alternative splicing transcript C is generated from B by removing the cassette exon. A decoy transcript D is generated from B by remove a random RNA sequence of length l, and a decoy transcript E is generated from C by adding a random exon of length l. By adding the heterozygous SNV, four heterozygous transcripts B’, C’, D’, and E’ are generated from B, C, D, and E, respectively.