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Workflow of the FIE-FTICR MS-based platform for metabolomics. Plasma samples were first extracted and then directly injected into FTICR MS without LC separation for detection and label-free quantification of metabolite features. Metaboscape was used for statistical analysis and SmartFormula annotation. METLIN provided annotations by accurate mass matching.Targeted MS/MS was performed for highly abundant and statistically significant features.
