Figure 9.

Mineral Deposits by Von Kossa Staining

(A to D) Images of mineral deposition, indicated by arrows, in (A and C) rTEHV and (B and D) aTEHVs after 6- and 12-month follow-up. Inset shows representative examples of micro deposits, as variant A; micro noduli (insets A2, B2, D1, and D2) or variant B; affected extracellular matrix components (insets A1, B1, C1) and macro size mineral deposits (C2). Individual data of mineral deposition after (E) 1, (F) 6, or (G) 12 months implantation, presented as color-code expression (green: not present and red: present) on the presence of micro (variant A and B) or macro size minerals deposits for different valve locations (hinge, belly, and tip). (Scale bar tile scans, 500 μm; scale bars insets, 50 μm). Abbreviations as in Figure 2.