Figure 2.

HES4 is not sufficient to inhibit B-cell development. Flow cytometric analysis (A, C) and absolute cell numbers (B, D) of control, ICN1-, HES1- and HES4- transduced CD34⁺Lin^– CB progenitors cultured on the MS-5 feeder (Notch-OFF) for 3 weeks in the presence of the B-lineage supporting cytokines IL-7 and SCF, showing the development of CD19⁺HLA-DR⁺ B-lineage cells (A-B) and CD7⁺CD5⁺ T-lineage cells (C-D). Data shows the average of six independent experiments and error bars indicate SEM. *P<0.05 (non-parametric paired Wilcoxon test); ns: not significant; ND: not detectable. Flow cytometry plots shown are representative for six independent experiments.