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Simulated performance of the end-to-end optimized DeepDOF microscope in a test set vs. two conventional baselines and a cubic-mask-enabled computational microscope in a layered model. (A) The DeepDOF microscope design shows superior performance across the entire defocus range. (B) Example reconstructed images from the test set showing sparsely (Upper) and densely (Lower) packed nuclei. In both FOVs, the DeepDOF microscope better resolves adjacent nuclei.
