Fig. 3.

HyPR-seq in mouse kidney cells detects changes in cell frequencies in diabetes. (A) UMAP visualization of HyPR-seq data for 31 genes in 14,288 BTBR wt/wt cells and 14,837 BTBR ob/ob cells identifies 11 distinct cell types. Endo, endothelial cell; PCT, proximal-convoluted tubule; PEC, parietal epithelial cell; TAL/DCT, thick ascending limb/distal convoluted tubule cell. (B) Dot plot displays kidney cell types with the fraction of cells in each subtype expressing each gene (circle size) and the relative level of gene expression (color). (C) Change in cell proportions in diabetic versus wild-type mice. Error bars represent the SD of the ratio from two biological replicates (Materials and Methods).