Table 2.

AS₂ for gene expression in P. aeruginosa PAO1

Model	AS2, %
	Combined in vivo	Sputum	Chronic wound/burn
Resampled in vivo	100	100	100
Mouse wound model	92	84	88
SCFM2	91	82	88
CFECs	86	79	82
RPMI	85	74	82
RPMI/serum	84	72	81
RSV-CFECs	83	75	81
Mops succinate	80	72	76
LB	77	69	76
MHB	72	65	70

AS₂ for gene expression in P. aeruginosa PAO1 grown in MHB, RPMI + 5% MHB (RPMI), and RPMI + 5% MHB + 20% human serum (RPMI/serum) and previously published in vitro models, when compared to in vivo transcriptomes in CF sputum and chronic wound (31), showed greater similarity between the host-mimicking conditions used in this study and in vivo samples cf. nutrient-rich laboratory media. The percentage of genes within 2 SDs (AS₂) of the mean expression for each gene in CF sputum (total, 2,437 genes) and chronic wound samples (957 genes) or a combined in vivo dataset (835 genes) (31) was determined with z-score calculations using the mean of normalized read counts.