Table 3.

AS₂ for the expression of genes in PseudCap (32) functional classes

Functional category	AS2, %
	RPMI	RPMI/serum	LB
Adaptation and protection	75	75	73
Amino acid metabolism and biosynthesis	84	78	80
Antibiotic resistance and susceptibility	79	79	64
Biosynthesis of cofactor prosthetic groups and carriers	86	84	84
Cell division	94	94	88
Cell wall LPS capsule	87	91	89
Central intermediary metabolism	80	80	76
Chaperone and heat shock	100	100	94
Chemotaxis	89	67	78
Energy metabolism	75	73	72
Fatty acid and phospholipid metabolism	90	86	86
Membrane	83	80	73
Motility	90	81	81
Nucleotide biosynthesis and metabolism	93	93	75
Protein secretion and export	93	100	87
Putative enzyme	90	88	86
Secreted factors	100	100	67
Transcription, RNA processing and degradation	91	91	78
Transcriptional regulator	73	64	59
Translation and posttranslational modification	90	88	71
Transport small molecules	84	81	69
Two-component regulators	95	89	68

AS₂ for the expression of genes in PseudCap (32) functional classes where transcriptomes from RPMI + 5% MHB (RPMI) and RPMI + 5% MHB + 20% human serum (RPMI/serum) outperformed transcriptomes in LB compared to in vivo transcriptomic data from CF sputum samples and chronic wound. The AS₂ represents the percentage of genes within 2 SDs of the mean expression levels in CF sputum and chronic wound samples as determined with z-score calculations using the mean of normalized read counts. Functional categories that performed as well or better than the other in vitro models Mops succinate and SCFM2 are bolded.