Fig. 4.

(A) The ¹H,¹⁵N-correlation spectra of MLKL executioner domain in the presence of 12 mM NM and 50 µM IP6 directly after adding 50 µM IP6 (black) and after 45.7 h at 298 K (red). (B) The ¹H,¹⁵N-correlation spectra of the MLKL executioner domain in the presence of 12 mM NM, 50 µM IP6, and 500 µM Cpd 3 directly after adding 50 µM IP6 (black) and after 47.7 h at 298 K (green). Assignments of selected peaks are indicated. (C and D) Peak intensity from the time course of ¹H,¹⁵N-correlation spectra for Met62 (C) and for the activated, α-helix 6 detached state of Ala141 (D) for the MLKL executioner domain under the condition described in A (orange) and with additionally 500 µM Cpd 3 (blue). Solid black lines indicate biexponential fitting curves for the time course in presence of NM and IP6. Act., active; inact, inactive.