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Multiple substrates feed into gluconeogenesis. Alanine, lactate, glycerol, and glutamine can generate glucose. Glycerol enters gluconeogenesis through conversion into dihydroxyacetone phosphate (DHAP), a reaction catalyzed by glycerol 3-phosphate dehydrogenase. Alanine, lactate, and glutamine have to be converted into oxaloacetate, which enters gluconeogenesis through conversion into PEP by phosphoenolpyruvate carboxykinase.
