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. 2021 Jan 1;35(1-2):133–146. doi: 10.1101/gad.344556.120

Figure 5.

Figure 5.

Hepatic FGF21 is required for the metabolic actions of JNK signaling in adipocytes. (A–C) ADWT, ADKO, and ADKOΔFgf21 mice were treated with AAV8-TBG-Cre and fed a HFD (16 wk). The expression of Fgf21, Fgfr1, and Klb by IngF and liver was measured by TaqMan assays (mean ± SEM; n = 5∼8). (*) P < 0.05; (**) P < 0.01. (D) The blood concentration of FGF21 was measured by ELISA (mean ± SEM; n = 6∼7). (**) P < 0.01; (***) P < 0.001. (E–G) Tolerance tests for glucose (GTT), pyruvate (PTT), and insulin (ITT) were performed (mean ± SEM; n = 6∼7). (*) P < 0.05; (**) P < 0.01; (***) P < 0.001. (H) The fasted blood glucose concentration was measured (mean ± SEM; n = 6∼7). (**) P < 0.01. (I) The blood concentration of insulin was measured by ELISA (mean ± SEM; n = 6∼7). (*) P < 0.05; (**) P < 0.01. (J) Sections prepared from IngF and liver were stained with H&E. Scale bar, 100 µm. (K) RNA isolated from IngF was examined using TaqMan assays to measure mRNA expression and presented as a heat map (n = 6∼8).