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. 2020 Jul 22;177(2):405–419. doi: 10.1093/toxsci/kfaa118

Figure 7.

Figure 7.

Target inhibition of AZD7762 on ovarian follicle. A, Representative images of follicles in bright field and follicular cell apoptosis after follicles were treated with AZD7762 and AZD8542 exposure at 10 µM for 24 h in vitro. Blue: DAPI; green: DNA fragmentation revealed by TUNEL staining. Scale bar: 100 μm. White squares indicated the TUNEL staining of oocytes. N = 5–10 follicles for each experimental group and 3 replicates were performed. B and C, Effect of 3 Chk1 inhibitors (Rabusertib at 2 μM, CHIR-124 at 0.2 Μm, and MK-8776 at 20 μM) and 1 Chk2 inhibitor (BML-277 at 5 µM) on follicle survival (B) and development (C) during encapsulated in vitro follicle growth. Error bar: SD; ***p < .001 compared with control group. N = 8–12 follicles in each experimental group and 3 replicates were performed.