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. 2021 Jan 2;40(3):477–484. doi: 10.1007/s10096-020-04106-0

Table 1.

Cell lines tested for their susceptibility to SARS-CoV-2

Cell lines Species of origin Cell types Characteristics References Culture medium Culture conditions
Aa23 Aedes albopictus Larva cells Morphology: epithelial, culture properties: adherent ATCC® CCL-125™ L15 Leibovitz + 10% FBS + 8% tryptose phosphate 28 °C
C6/36 Aedes albopictus Larva cells Morphology: epithelial-like, culture properties: adherent ATCC® CRL-1660™ L15 Leibovitz + 10% FBS + 8% tryptose phosphate 28 °C
S2 Drosophila melanogaster Embryo cells Morphology: macrophage-like, culture properties: semi-adherent and suspension Thermo R69007 Schneider medium + 10% FBS 28 °C
ISE6 Ixodes scapularis Embryo cells Morphology: neuron-like, culture properties: adherent ATCC® CRL-11974 L15B + 10% FBS 28 °C
IPL-LD-65Y Lymantria dispar Larvae cells Morphology: fibroblastic like, culture properties: semi-adherent and suspension ACC 181 (DSMZ) TC-100 + 101% FBS 28 °C
BGM Cercopithecus aethiops Kidney cells Morphology: epithelial-like, culture properties: adherent ECACC 90092601 MEM + 10% FBS 37 °C 5% CO2
VERO/hSLAM Cercopithecus aethiops Kidney cells Morphology: fibroblastic, culture properties: adherent 04091501-1VL MEM + 5% FBS + 37 °C 5% CO2
MA104 Cercopithecus aethiops Kidney cells Morphology: epithelial, culture properties: adherent ATCC® CRL-2378.1™ MEM + 10% FBS 37 °C 5% CO2
VERO 81 Cercopithecus aethiops Kidney cells Morphology: epithelial, culture properties: adherent ATCC® CCL-81™ MEM + 4% FBS 37 °C 5% CO2
VERO E6 Cercopithecus aethiops Kidney cells Morphology: epithelial, culture properties: adherent ATCC® CRL-1586™ MEM + 10% FBS 37 °C 5% CO2
LLC-MK2 Macaca mulatta Kidney cells Morphology: epithelial, culture properties: adherent ATCC® CCL-7™ M199 + 1% FBS 37 °C 5% CO2
HT-29 Homo sapiens Cells from colorectal adenocarcinoma Morphology: epithelial, culture properties: adherent ATCC® HTB-38™ DMEM/F12 + 10% FBS 37 °C 5% CO2
Caco-2 Homo sapiens Cells from Colorectal adenocarcinoma Morphology: epithelial, culture properties: adherent ATCC® HTB-37™ DMEM + 10% FBS + 1%AA 37 °C 5% CO2
HELA Homo sapiens Cervix cells from adenocarcinoma Morphology: epithelial, culture properties: adherent ATCC® CCL-2™ MEM + 10% FBS 37 °C 5% CO2
HCT-8 Homo sapiens Colon cells from ileocecal colorectal adenocarcinoma Morphology: epithelial, culture properties: adherent ATCC® CCL-244™ RPMI + 10% FBS 37 °C 5% CO2
HEP-2 Homo sapiens HeLa derived cell line from laryngeal epidermoid carcinoma Morphology: epithelial, culture properties: adherent ATCC® CCL-23™ MEM + 5% FBS + 1% AA 37 °C 5% CO2
ECV304 Homo sapiens Cells from human cord/urinary bladder carcinoma Morphology: epithelial/endothelial, culture properties: adherent ATCC® CRL-1998™ RPMI + 10% FBS 37 °C 5% CO2
HL-60 Homo sapiens Promyeloblast cells from human peripherical blood from acute promyelocytic leukemia Morphology: lymphoblast-like, culture properties: suspension ATCC® CCL-240™ RPMI + 10% FBS 37 °C 5% CO2
MRC5 Homo sapiens Cells from lung Morphology: fibroblast, culture properties: adherent ATCC® CCL-171™ MEM + 10% FBS 37 °C 5% CO2
THP1 Homo sapiens Cells from peripheral blood from acute monolytic leukemia Morphology: monocytes, culture properties: suspension ATCC® TIB-202™ RPMI + 10% FBS 37 °C 5% CO2
BHK21 Mesocricetus auratus Kidney cells Morphology: fibroblast, culture properties: adherent ATCC® CCL-10™ MEM + 4% FBS 37 °C 5% CO2
McCoy Mus musculus Unknown Morphology: fibroblast, culture properties: adherent ATCC® CRL-1696™ MEM + 4% FBS 37 °C 5% CO2
L929 Mus musculus Cells from subcutaneous areolar and adipose Morphology: fibroblast, culture properties: adherent ATCC® CCL1™ MEM + 4% FBS 37 °C 5% CO2
P388D1 Mus musculus Cells from lymphoma Morphology: lymphoblast, culture properties: suspension ATCC® CCL-46™ MEM + 10% FBS 37 °C 5% CO2
RAW 264.7 Mus musculus Cells from Abelson murine leukemia virus-induced tumor Morphology: monocyte/macrophage, culture properties: adherent ATCC® TIB-71™ MEM + 10% FBS + AA 37 °C 5% CO2
BA 886 Bos taurus Cells from bovine aorta Morphology: endothelial, culture properties: adherent [15] DMEM/F12 + 10% FBS 37 °C 5% CO2
MDCK Canis familiaris Cells from kidney Morphology: epithelial, culture properties: adherent ATCC® CCL-34™ MEM + 10% FBS 37 °C 5% CO2
DH82 Canis familiaris Cells from malignant histiocytosis Morphology: macrophage-like, culture properties: adherent ATCC® CRL-10389™ MEM + 10% FBS 37 °C 5% CO2
OA3.Ts Ovis aries Testis cells Morphology: epithelial, culture properties: adherent ATCC® CRL-6546™ DMEM + 10% FBS 37 °C 5% CO2
MDOK Ovis aries Kidney cells Morphology: epithelial, culture properties: adherent ATCC® CRL-1633™ MEM + 10% FBS + 1% AA + 1% pyruvate 37 °C 5% CO2
R05T Rousettus aegyptiacus Head of fetus Morphology: epithelial, culture properties: adherent Bei resources NR-49169 DMEM/F12 + 10% FBS 37 °C 5% CO2
R06E Rousettus aegyptiacus Fetus cells Morphology: epithelial, culture properties: adherent Bei resources NR-49168 DMEM/F12 + 10% FBS 37 °C 5% CO2
TB1 Lu Tadarida brasiliensis Lung cells Morphology: epithelial, culture properties: adherent ATCC® CCL-88™ DMEM + 10% FBS 37 °C 5% CO2
XTC-2 Xenopus laevis Tadpole cells Morphology: epithelial, culture properties: adherent CellBank Riken® RCB0771 L15 Leibovitz + 5% FBS + 8% tryptose phosphate 28 °C

FBS, fetal bovine serum; AA, nonessential amino-acids