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. Author manuscript; available in PMC: 2021 Jan 2.
Published in final edited form as: Biochemistry. 2019 Oct 17;58(43):4361–4373. doi: 10.1021/acs.biochem.9b00739

Figure 4.

Figure 4.

ThT aggregation assays for htt-exon1(46Q) aggregated in the presence of (A) POPC or (B) TBLE lipid vesicles. The htt-exon1(46Q) concentration was 20 μM. Curcumin and EGCG were added at a 5:1 small molecule:htt molar ratio. The initial rate of aggregation in the presence of (C) POPC or (D) TBLE vesicles was made relative to the aggregation rate of htt with the lipid vesicles in the absence of the small molecules. Finally, the relative maximum fluorescence values of all conditions in the presence of (E) POPC or (F) TBLE lipid vesicles at the end of the 18 h kinetic run are compared. Analyses shown in panels C–F were determined as averages over all trials (shown in Figure S2). Error bars are provided for every sixth data point (30 min) and represent the standard error of the mean. One asterisk represents a p value of <0.05, and two asterisks represent a p value of <0.01.