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. Author manuscript; available in PMC: 2021 Jan 2.
Published in final edited form as: Biochemistry. 2019 Oct 17;58(43):4361–4373. doi: 10.1021/acs.biochem.9b00739

Figure 5.

Figure 5.

AFM analysis of the impact of curcumin or EGCG on htt-exon1(46Q) aggregation (5:1 small molecule:htt ratio) in the presence of POPC lipid vesicles (20:1 lipid:protein ratio). (A) Representative AFM images of 20 μM htt-exon1(46Q) incubated with POPC with no small molecules, with curcumin, or with EGCG as a function of time. The color map is the same for all images. (B) Image analysis of the number of oligomers per unit area as a function of time. (C) Height histograms of oligomers observed in AFM images as a function of time. (D) Image analysis of the number of fibrils per unit area as a function of time. Arrows indicate that no fibrils were observed. (E) Height histograms of fibrils observed in AFM images as a function of time. As fibrils were not observed for htt-exon1(46Q) incubations with POPC and EGCG, this condition is not presented in the fibril histograms. For panels B and D, error bars represent the standard deviation, one asterisk represents a p value of <0.05, and two asterisks represent a p value of <0.01 using a Student’s t test.