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. 2021 Jan 1;12(3):771–789. doi: 10.7150/jca.49680

Figure 1.

Figure 1

MiR-1254 is upregulated in HCC cell lines and tissues. (A) MiR-1254 expression in paired human HCC and adjacent normal tissues from The Cancer Genome Atlas (TCGA) database. (B) MiR-1254 expression in 50 paired human HCC and adjacent normal tissues from patients with HCC. (C) MiR-1254 expression in HCC cell lines and normal LO2 cells. (D) Expression of miR-1254 in HCC and adjacent normal tissues detected by fluorescence in situ hybridization (FISH) assay (scale bars: 50 µm). (E and F) Lentivirus overexpressing miR-1254 (pre-miR-1254) or with short hairpin RNA targeting miR-1254 (miR-1254-inhibitor) were used to transfect Hep3B and Huh-7 cells. Cells with empty lentiviral vectors were negative controls (NC). RT-qPCR was used to analyze expression of miR-1254 after transfection; All experiments were performed three times and data are mean ± SD. *P<0.05, **P<0.01, ***P<0.001.