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. 2021 Jan 1;12(3):771–789. doi: 10.7150/jca.49680

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MiR-1254 promotes HCC cell migration, invasion, and EMT in vitro. (A and B) Transwell assays demonstrated migration and invasion of Hep3B and Huh-7 cells with overexpressed or knocked down miR-1254. (C-F) Wound-healing assays were performed in Hep3B and Huh-7 cells with overexpressed or knocked down miR-1254. (G) Western blots examined levels of epithelial-mesenchymal transition (EMT)-related proteins. (H and I) Expression of EMT markers in Hep3B and Huh-7 cells with overexpressed or knocked down miR-1254 detected by immunofluorescence (red: E-cadherin, green: vimentin, blue: DAPI) (scale bars: 50 µm). Three independent experiments were performed for each group. All data are means ± SD. *P<0.05, **P<0.01, ***P<0.001.

MiR-1254 promotes HCC cell migration, invasion, and EMT in vitro. (A and B) Transwell assays demonstrated migration and invasion of Hep3B and Huh-7 cells with overexpressed or knocked down miR-1254. (C-F) Wound-healing assays were performed in Hep3B and Huh-7 cells with overexpressed or knocked down miR-1254. (G) Western blots examined levels of epithelial-mesenchymal transition (EMT)-related proteins. (H and I) Expression of EMT markers in Hep3B and Huh-7 cells with overexpressed or knocked down miR-1254 detected by immunofluorescence (red: E-cadherin, green: vimentin, blue: DAPI) (scale bars: 50 µm). Three independent experiments were performed for each group. All data are means ± SD. *P<0.05, **P<0.01, ***P<0.001.