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. 2021 Jan 1;12(3):771–789. doi: 10.7150/jca.49680

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PAX5 is a functional target of miR-1254 in HCC. (A) RT-qPCR was used to measure mRNA of PAX5 in HCC cell lines and normal L02 cells. (B) RT-qPCR was used to measure mRNA of PAX5 in 50 paired human HCC and adjacent normal tissues. (C) Immunohistochemistry (IHC) detected expression of PAX5 in HCC and adjacent non-tumor tissues. (D) Target Scan was used to predict potential miR-1254 binding sites in wild-type (WT) PAX5 3'-UTR or mutant-type (MUT) PAX5 3'-UTR. (E) mRNA levels of PAX5 in over-expressed miR-1254 Hep3B and Huh-7 cells with overexpressed or knocked down miR-1254. Cells transfected with empty lentiviral vectors were negative controls (NC). (F and G) PAX5 protein in Hep3B and Huh-7 cells with with overexpressed or knocked down miR-1254. (H) Dual-luciferase reporter assays to investigate effects of miR-1254 expression on activities of WT and MUT PAX5 3'-UTR. (I) Negative correlation between the levels of miR-1254 and PAX5 expression in HCC tissues. All data are means ± SD. *P<0.05, **P<0.01, ***P<0.001.

PAX5 is a functional target of miR-1254 in HCC. (A) RT-qPCR was used to measure mRNA of PAX5 in HCC cell lines and normal L02 cells. (B) RT-qPCR was used to measure mRNA of PAX5 in 50 paired human HCC and adjacent normal tissues. (C) Immunohistochemistry (IHC) detected expression of PAX5 in HCC and adjacent non-tumor tissues. (D) Target Scan was used to predict potential miR-1254 binding sites in wild-type (WT) PAX5 3'-UTR or mutant-type (MUT) PAX5 3'-UTR. (E) mRNA levels of PAX5 in over-expressed miR-1254 Hep3B and Huh-7 cells with overexpressed or knocked down miR-1254. Cells transfected with empty lentiviral vectors were negative controls (NC). (F and G) PAX5 protein in Hep3B and Huh-7 cells with with overexpressed or knocked down miR-1254. (H) Dual-luciferase reporter assays to investigate effects of miR-1254 expression on activities of WT and MUT PAX5 3'-UTR. (I) Negative correlation between the levels of miR-1254 and PAX5 expression in HCC tissues. All data are means ± SD. *P<0.05, **P<0.01, ***P<0.001.