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. 2021 Jan 1;12(3):860–873. doi: 10.7150/jca.48141

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The influences of upregulation and downregulation of DTX3 expressions on migration and invasion of PTC cells. (A) DTX3 relative downregulated protein expression levels were measured by western blot using anti-DTX3 antibody. GAPDH was used as a loading control. Lower panel: Integrated optical density (IOD) of DTX3 protein was presented as relative expression level after normalization with internal control. (B) DTX3 relative upregulated protein expression levels were measured by western blot using anti-DTX3 antibody. GAPDH was used as a loading control. Lower panel: IOD of DTX3 protein was presented as relative expression level after normalization with internal control. (C) DTX3 relative upregulated and downregulated mRNA expression levels were measured by qRT-PCR. (D) The migration abilities affected by upregulation and downregulation of DTX3 were measured by wound healing assay (100× magnification). (E) The invasion abilities affected by upregulation and downregulation of DTX3 were measured by Matrigel-coated transwell invasion assay (200× magnification). *P < 0.05, n.s means not significant.