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. 2021 Jan 1;11(4):1763–1779. doi: 10.7150/thno.47057

Figure 2.

Figure 2

MiR-30b-3p is functionally overexpressed in hypoxic EVs. (A) 77 miRNAs were significantly different between normoxic EVs and hypoxic EVs. (B) qRT-PCR analysis of miR-30b-3p in EVs from GSC664 and GSC712 cells under normoxia or hypoxia. Six replicates per sample, three independent experiments per sample. (C) Representative confocal microscopy images of EV-miR-30b-3p internalization by TC664 and TC712 cells. Scale bar, 10 µm. (D) qRT-PCR analysis of miR-30b-3p in TC664 and TC712 cells treated with different EVs. Six replicates per sample, three independent experiments per sample. (E) Colony formation assay of TC664 and TC712 cells treated with different EVs. Six replicates per group, three independent experiments per group. (F) Apoptotic rates of TC664 and TC712 cells treated with different EVs were measured by flow cytometry. Six replicates per group, three independent experiments per group. (G) Apoptotic rates of TC664 and TC712 cells treated with different EVs were measured by TUNEL analysis. Six replicates per group, three independent experiments per group. (H) Western blot analysis of caspase-3 (full length and cleaved) in TC664 and TC712 cells treated with different EVs. Three replicates per group, three independent experiments per group, **P < 0.01.